Chithunzithunzi cha Lactoperoxidase

Lactoperoxidase (LPO), Lomwe likupezeka mu zopangitsa malovu ndi mammary, ndi chinthu chofunika kwambiri mmene mthupi zofunika kukhalabe wabwino m'kamwa thanzi. Udindo kwambiri kuposa lactoperoxidase ndi oxidize thiocyanate ayoni (SCN-) zopezeka mu malovu pamaso wa hydrogen peroxide chifukwa mu mankhwala Onetsani antimicrobial. LPO zopezeka mkaka Bovine wakhala ntchito zachipatala, chakudya, ndi zodzoladzola mafakitale chifukwa n'zofanana ndi zinchito ndi structural kwa enzyme anthu.

Zinthu zamakono zogwiritsira ntchito zaukhondo zikulemekezedwa ndi njira ya lactoperoxidase kuti ipereke njira yabwino kwina lodziwika bwino ngati dzino la fluoride. Chifukwa cha ntchito waukulu wa lactoperoxidase yowonjezera, Kufunika kwake chawonjezeka kwambiri zaka, ndipo mpaka pano kukula.
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Lactoperoxidase ndi chiyani?

Lactoperoxidase imangokhala enzyme ya peroxidase yopangidwa kuchokera ku mucosal, mammary, ndi salivary gland, yomwe imagwira ntchito ngati anti-bacterial agent. Mwa anthu, ndi lactoperoxidase enzyme ndi ndiyotengera ndi LPO mbewu. Enzyme imeneyi imakonda kupezeka mwa anyama, kuphatikiza anthu, mbewa, bovine, ngamila, njati, ng'ombe, mbuzi, Ilama, ndi nkhosa.

Lactoperoxidase Limagwira:

LPO ndi kwambiri ogwira antimicrobial wothandizira. Kugwiritsa ntchito kwa Lactoperoxidase, chifukwa chake, kutengera izi. ntchito Lactoperoxidase Izi zingamutha- anapeza makamaka chakudya kuteteza njira ophthalmic ndi zolinga zodzikongoletsera. Komanso, lactoperoxidase ufa lakhala likugwiritsidwa ntchito bala ndi mano chithandizo. Komanso, LPO ndi ogwira odana ndi tizilombo ndi odana ndi chotupa wothandizira. Zomwe takambirana pansipa ndizogwiritsa ntchito lactoperoxidase:

i. Khansara ya m'mimba

khansa Lactoperoxidase mphamvu kasamalidwe amagwirizana ndi mphamvu yake oxidize estradiol. makutidwe ndi okosijeni Zimenezi zimabweretsa nkhawa zobwera chifukwa cha okosijeni maselo khansa. Lactoperoxidase ntchito pano n'chakuti unyolo zochita wopita ku mowa mpweya ndi kudzikundikira hydrogen peroxide okhudza maselo ambiri. Chifukwa cha izi, LPO bwino imapha zotupa m'matro. Komanso, macrophages poyera kuti LPO adamulowetsa kuwononga maselo a khansa, kuzipha.

ii. Anti-bakiteriya zotsatira

Enzulopu ya LPO imagwira ntchito ngati gawo lachilengedwe la chitetezo chopanda chitetezo cha m'thupi mwa zolengedwa ndipo imayambitsa makupidwe a oxocation wa thiocyanate ion mu antibacterial hypothiocyanate. LPO akhoza ziletsa kukula osiyanasiyana waukulu tizilombo kudzera ndi anachita enzymatic zimene zimaphatikizapo ayoni thiocyanate ndi hydrogen peroxide monga cofactors. ntchito antimicrobial wa LPO ndi mfundo pa mapangidwe ayoni hypothiocyanite mwa kutsegula kwa michere. Hypothiocyanite ion imatha kuchita ndi bacteria. Iwo chifukwa chisokonezo pa ntchito kwa michere kagayidwe kachakudya makamaka. Lactoperoxidase amapha mabakiteriya galamu alibe ndiponso kupewa kukula ndi chitukuko cha tizilombo galamu HIV.

iii. Lactoperoxidase mu zodzoladzola

Kuphatikiza kwa lactoperoxidase ufa, shuga, thiocyanate, iodide,

ndi shuga oxidase, ndipo amadziwika mogwira zodzoladzola atetezedwe.Lactoperoxidas-02

iv. Lactoperoxidase mu mkaka Kusungidwa

Kutha kwa lactoperoxidase pakukonzanso mkaka wabwino waiwisi kwa nthawi yayitali kwakhazikitsidwa m'magawo angapo ndipo maphunziro oyesera omwe amachitika m'malo osiyanasiyana. Lactoperoxidase usawonongeke angagwiritsidwe ntchito kusunga mkaka yaiwisi kuwapeza mitundu yosiyanasiyana. Mtima njira ndi kumatengera zifukwa angapo. Zinthu izi zimaphatikizira kutentha kwa mkaka munthawi yamankhwala, mtundu wa kuipitsidwa kwa tizilombo, komanso kuchuluka kwa mkaka.

Lactoperoxidase ochitachita zotsatira bacteriostatic mu mkaka nyama za yaiwisi. deta Research ndi zinachitikira kwenikweni kumapeto zimasonyeza kuti lactoperoxidase angagwiritsidwe ntchito malire kutentha kwa (15-30 madigiri Celsius) amatchulidwa mu malangizo mabuku a 1991. Pamapeto osachepera lonse kutentha, maphunziro osiyana bwanji kuti kutsegula kwa lactoperoxidase akhoza sachedwa psychrotrophic a mkaka mabakiteriya kukula ndipo motero sachedwa spoilage mkaka kwa masiku poyerekeza refrigeration yekha. Ndi chofunika kwambiri kudziwa kuti cholinga cha ntchito lactoperoxidase si kuti otetezedwa mkaka mowa koma kusunga choyambirira zidazo.

Kuchita ukhondo kupanga mkaka ndi ofunika kwambiri efficacy wa lactoperoxidase ndi microbiological khalidwe mkaka. Chitetezo ndi kutsitsimuka kwa mkaka akhoza Zimapezeka ndi osakaniza kutentha mankhwala a mkaka ndi makhalidwe abwino ukhondo osadalira pa ntchito lactoperoxidase.

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v. Ntchito zina

Kafukufuku wasonyeza kuti kuwonjezera ndi zotsatira mavairasi oyambitsa, Lactoperoxidase Mukhozanso maselo kuteteza ziweto kumawononga zosiyanasiyana ndi peroxidation, ndipo izo ndi yofunika zikuluzikulu za chitetezo yolimbana tizilombo toyambitsa matenda mu dongosolo m'mimba mwa makanda obadwa kumene.

Lactoperoxidase System

ndi Lactoperoxidase System ndi chiyani?

Lactoperoxidase system (LPS) wapangidwa zigawo zitatu, monga lactoperoxidase, hydrogen peroxide, ndi thiocyanate (SCN¯). Dongosolo Lactoperoxidase kugwira ntchito odana ndi bakiteriya kokha pamene zigawo zitatu izi ntchito pamodzi. Mu ntchito zenizeni m'moyo, ngati ndende za chinthu makamaka mu dongosolo sikokwanira, izo ziyenera kuwonjezeredwa onetsetsani zotsatira odana ndi bakiteriya, lomwe limatchedwa LPS kutsegula. Pakati pawo, lactoperoxidase ndende sayenera zosakwana 0.02 U / mL.

Zachilengedwe lactoperoxidase ndende mu mkaka Bovine ndi 1.4 U / mL, amene akhoza kukwaniritsa mfundo yofunika imeneyi. SCN¯ ndi monzama zikupezeka secretions ndi zimakhala nyama. Mu mkaka, anthu ambiri thiocyanate ndi zochepa monga 3-5 μg / mL. Ichi ndi chinthu Kuchepetsa kwa Lactoperoxidase dongosolo ntchito. Anthu ena amanena kuti thiocyanate anafunika yambitsa dongosolo lactoperoxidase ali mozungulira 15 μg / mL kapena kuposa. N'chifukwa chake tiyenera kuwonjezera ichi thiocyanate exogenous kuti yambitsa dongosolo lactoperoxidase. The hydrogen peroxide zili mu mkaka, amene wakhala extruded, ndi 1-2 kokha μg / ml, ndi kutsegula kwa LPS ayenera 8-10 μg / mL wa hydrogen peroxide. Ndicho chifukwa hydrogen peroxide ayenera kunja aperekedwe.

Dongosolo lactoperoxidase amagwira ntchito yofunika kwambiri mu chitetezo titadziwa, zikhoza mabakiteriya mu mkaka ndi secretions mucosal ndipo mwina ntchito achire.

Mu chakudya ndi zaumoyo mankhwala, kuwonjezera kapena augmentation a dongosolo lactoperoxidase zina ntchito kulamulira mabakiteriya.

Kodi ntchito?

The LPS imakhala kupanga pawiri odana ndi bakiteriya ku SCN¯catalysed ndi LPO pamaso pa hydrogen peroxide. The lactoperoxidase anati ntchito antimicrobial mwachibadwa opezeka madzi angapo thupi monga madzi chapamimba, misozi, ndi malovu. The awiri m'nyanjazi zofunika kwa dongosolo antimicrobial, amene hydrogen peroxide ndi thiocyanate, alipo mu mkaka woipa osiyana, malingana ndi mtundu wa nyama ndi chakudya anapatsidwa.

Mu mkaka atsopano, ntchito antimicrobial ndi ofooka ndi iwowa zochepa kwambiri monga 2 hours kuyambira mkaka lili yekha milingo suboptimal wa peroxide pa hydrogen ndi ion thiocyanate. Thiocyanate anawonjezera amene oxidized mu hypothiocyanite 2 elekitironi anachita ololera

ntchito Thiocyanate monga cofactor kwa dongosolo Lactoperoxidase. Chifukwa cha zimenezi, chiwerengero cha sulfhydryls oxidized mu okwana zimadalira pa ion thiocyanate bola ngati

  1. Thiol moiety akupezeka
  2. Thiocyanate si wotopa
  • Zokwanira hydrogen peroxide alipo
  1. Thiocyanate sanayambebe mbali ndi kwa amino asidi onunkhira

Chifukwa, thiocyanate reactivates zotsatira odana ndi bakiteriya a dongosolo lactoperoxidase mu mkaka atsopano. Izi chimafikira alumali moyo wa mkaka atsopano m'mikhalidwe otentha kwa maola asanu ndi atatu.

Lactoperoxidase Phunziro pankhaniyi / Ntchito

i. Odana ndi tizilombo tating'onoting'ono Action

Odana ndi tizilombo tating'onoting'ono ntchito dongosolo lactoperoxidase tikaona kanthu bactericidal ndi bacteriostatic ena tizilombo amapezeka mu mkaka yaiwisi. njira yake bactericidal ntchito mu gulu thiol pa maselo tating'onoting'ono 'plasma Kakhungu ndi oxidized. Izi zotsatira mu chiwonongeko plasma Kakhungu dongosolo la wopita ku kutayikira la polypeptides, ayoni potassium ndi amino zidulo. The kutengedwa wa purines ndi pyrimidines, shuga, ndi kwa amino asidi ndi maselo imathetsa. Kaphatikizidwe DNA, RNA, komanso mapulotini amagwiritsidwa imathetsa.

mabakiteriya osiyana kusonyeza madigiri osiyana cha kuzindikira dongosolo lactoperoxidase. Galamu alibe mabakiteriya, monga nsomba, Pseudomonas, ndi Escherichia coli, akulephera ndi kuphedwa. Lactic mabakiteriya acid ndi Streptococcus pokhapokha imathetsa. Chiwonongeko cha bakiteriya zimenezi chifukwa lactoperoxidase dongosolo zimayambitsa kutayikira ena zakudya, kulepheretsa amawononga kudya zakudya, ndi kuwatsogolera izi kuchepa kapena imfa ya mabakiteriya.

ii. Chithandizo cha paradentosis, gingivitis, ndi kupha maselo chotupa

LPS amakhulupirira kuti agwire ntchito mankhwala a gingivitis ndi paradentosis. LPO lakhala likugwiritsidwa ntchito ndi pakamwa Muzitsuka kuchepetsa mabakiteriya m'kamwa ndi, chifukwa, chidulo opangidwa ndi mabakiteriya izi. conjugates oteteza a dongosolo lactoperoxidase ndi shuga oxidase asonyeza kuti azigwira kuwononga ndipo potsatira kupha maselo chotupa mu m'galasi. Komanso, macrophages poyera dongosolo lactoperoxidase adamulowetsa kuwononga ndi kupha maselo a khansa.

iii. Kusamalira Mwamlomo

Osiyana matenda maphunziro kufotokoza mphamvu ya LPS mu mankhwala otsukira mano akhala zalembedwa. Pambuyo njira zina posonyeza, ntchito kuyeza experimental caries zinthu magawo, kuti amyloglucosidase lactoperoxidase mankhwala otsukira mano munali (γ-amylase) ali phindu mu chisamaliro m'kamwa. Michere monga shuga oxidase, lysozyme, ndi lactoperoxidase ndi anasamutsidwa mwachindunji mankhwala otsukira mano kuti pellicle lapansi.

Kukhala m'nyanjazi wa pellicle ndi michere imeneyi amapatsa yogwira catalytically. Komanso LPS kumakhudza opindulitsa kuteteza ndi caries oyambirira ubwana ndi kutsitsa chiwerengero cha madera amene anapanga ndi microflora cariogenic monga boosts ndende ya thiocyanate.

Ndi odwala xerostomia, lactoperoxidase otsukira mkamwa kwambiri kuposa poyerekeza ndi mankhwala otsukira mano fluoride pankhani mapangidwe zolengeza. Ntchito LPS sukhala periodontitis ndi caries. A kuphatikiza lactoperoxidase ndi lysozyme angagwiritsidwe ntchito pa matenda woyaka pakamwa matenda.

Pamene LPS ali pamodzi lactoferrin, kuphatikiza combats halitosis. Pamene LPS ali pamodzi lysozyme ndi lactoferrin, LPS amathandizanso akulitse zizindikiro xerostomia. Komanso, angakhale angelo ndi dongosolo lactoperoxidase kuthandiza kusintha zizindikiro khansa m'kamwa pamene malovu kupanga imathetsa chifukwa walitsa.

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iv. Utithandize chitetezo cha m'thupi

Lactoperoxidase ntchito antimicrobial umagwira ntchito yofunika kwambiri chitetezo cha m'thupi. Hypothiocyanite ndi chigawo zotakasika opangidwa ndi ntchito lactoperoxidase pa thiocyanate. hydrogen peroxide amapangidwa ndi Duox2 mapuloteni (wapawiri oxidase 2). Thiocyanate katulutsidwe odwala ndi enaake fibrosis ndi adatchithisira. Izi zimachititsa kuchepetsa kupanga za hypothiocyanite antimicrobial. Zimenezi chiopsezo apamwamba airway matenda.

The LPS kumabwezera mmbuyo helicobacter pylori mosabvuta. Koma anthu malovu lonse, LPS mapulogalamu chofooka zotsatira odana ndi bakiteriya. The LPS si kuukira DNA ndipo si mutagenic. Koma, pansi mikhalidwe, LPS mwina chifukwa maganizo pang'ono zobwera chifukwa cha okosijeni. Iwo imatsimikizirika kuti LPO pamaso pa thiocyanate lingachititse zotsatira cytotoxic ndi bactericidal wa hydrogen peroxide pa mikhalidwe, kuphatikizapo pamene H2O2 alipo mu zosakaniza zimene owonjezera thiocyanate.

Komanso, chifukwa cha katundu wake wamphamvu komanso zothandiza antibacterial ndi mkulu kutentha kukana, amagwiritsidwanso ntchito ngati wothandizira antibacterial yochepetsera madera bakiteriya mankhwala mkaka kapena mkaka ndipo monga chizindikiro cha mkaka kopitilira muyeso-pasteurization. Ndi mphamvu dongosolo lactoperoxidase, alumali moyo wa mkaka m'firiji yaiwisi akhoza imakula.

Ndipo hypothiocyanate umatulutsa lactoperoxidase angagwiritsidwe ntchito ziletsa nsungu simplex HIV ndi kamene kamawononga chitetezo m'thupi.

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Kodi palibe chovuta thanzi anthu ndi nyama?

zaka khumi ndi asanu a maphunziro kumunda kukhala ndi maiko olemera kunachitika ndipo anamuyesa mwa FAO / WHO JECFA (olowa Akatswiri Committee pa Food zina). Pambuyo maphunziro awa mozama ndi yokulirapo anamalizidwa, ntchito dongosolo lactoperoxidase mu kuteteza mkaka ovomerezedwa ndi FAO / WHO JECFA (Katswiri Committee pa Food zina). The akatswiri kutchulidwa njira imeneyi otetezedwa kwa onse thanzi la munthu ndi nyama.

The LPS ndi constituent chilengedwe cha madzi muzituluka ndi malovu anthu, choncho otetezeka pamene ntchito kutsatira malangizo Codex Alimentarius Commission a. Njira sikumangotikhudza nyama lactating zonse. Ichi ndi chifukwa chakuti mankhwala zichitike pokhapokha mkaka ndi yotengedwa ndi unyinji wa.

Kutsiliza

Tikaganizira zokambirana zathu kuti lactoperoxidase ndi dongosolo lactoperoxidase zimathandiza kwambiri ndi zothandiza kwambiri osiyanasiyana ofunsira. Ngati mukufuna kuti wangwiro ndi lactoperoxidase ugule kufufuza kapena mankhwala anu chitukuko, kuyang'ana patsogolo. Tili ndi luso ndondomeko lactoperoxidase malamulo zambiri mu nthawi yaifupi zotheka ndi zotumiza iwo ku US, Europe, Canada, ndi mbali zina angapo a dziko. Omasuka kulankhula nafe kuti mumve zambiri.

Zothandizira

  1. Jantschko, PG Furtmüller, M. Allegra neri Al, "Redox intermediates zomera ndi peroxidases zolengedwa zoyamwitsa: a poyerekeza chosakhalitsa-kayendedwe kuphunzira reactivity zawo kwa ena ofanana indole,". Archives a sayansi ndi Biophysics, Vol. 398, palibe. 1, pp. 12-22, 2002.
  2. Tenovuo jo (1985). "Dongosolo peroxidase mu secretions anthu." Mu Tenovuo Jo, Pruitt Km (eds.). Dongosolo Lactoperoxidase: umagwirira ndi tanthauzo kwachilengedwenso. New York: Dekker. p. 272.
  3. Thomas EL, Bozeman PM, Phunzirani DB: Lactoperoxidase: kapangidwe ndi othandizira katundu. Peroxidases mu Chemistry ndi Biology. Lolembedwa ndi: Everse J, Everse ke, Grisham MB. 1991, Boca Raton, FL. CRC Press, 123-142.
  4. Wijkstrom-Frei C, El-Chemaly S, Ali-Rachedi R, Gerson C, Cobas MA, Forteza R, Salathe M, Conner Ge (August 2003). "Lactoperoxidase ndi anthu airway khamu chitetezo". Am. J. Respir. Cell mol. Biol. 29 (2): 206-12.
  5. Mikola H, Waris M, Tenovuo J: chopinga wa nsungu simplex HIV mtundu 1, kupuma syncytial HIV ndi echovirus mtundu 11. peroxidase-kwaiye hypothiocyanite. Mavairasi oyambitsa res. 1995, 26 (2): 161-171.
  6. Haukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J (September 2004). "Tilinazo Helicobacter pylori kwa limagwirira titadziwa chitetezo, dongosolo lactoperoxidase, mu gawo lotetezedwa ndi malovu anthu lonse". Journal of Medical Microbiology. 53 (pt 9): 855-60.

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